Enzymes and Inhibitors
Research Products International Corp 4-Methylumbelliferyl-B-D-Galactopyranoside-6-Sulfate, Sodium Salt, 25 Milligrams
Fluorescent substrate for Morquio A Sulphatase.
- 4-Methylumbelliferone: <100PPM
Cytoskeleton, Inc. CELL PERMEABLE C3 TRANS FERASE
Cell Permeable Rho Inhibitor (C3 Trans based)) 1 x 20 ug
Bio IVT Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
Mouse CYP3A11 Low Reductase Bactosomes, 1nmol/vial
New England Biolabs, Inc. Cre Recombinase – 250 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
New England Biolabs, Inc. T4 Phage beta-glucosyltransferase – 500 units
T4 Phage -glucosyltransferase specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, making beta-glucosyl-5-hydroxymethylcytosine.
New England Biolabs, Inc. cAMP-dependent Protein Kinase (PKA), catalytic subunit – 500000 units
The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase. The recognition motif for phosphorylation by PKA is RRXS/TY, where Y tends to be a hydrophobic residue.
Takara Bio AB0481TAR HS DNA POL 250 UNIT
Enzyme; Polymerase; DNA; Speedstar HS; 250U; Used for high speed amplification and robust amplification of large DNA templates
Takara Bio TAQ HOT START PREMIX
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 100rxns; reduces background, increased specificity, permits room-temperature reaction assembly
New England Biolabs, Inc. Vaccinia Capping System – 400 units
Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.
New England Biolabs, Inc. cAMP-dependent Protein Kinase (PKA), catalytic subunit – 100000 units
The catalytic subunit of cAMP-dependent Protein Kinase (PKA) is a serine/threonine protein kinase. The recognition motif for phosphorylation by PKA is RRXS/TY, where Y tends to be a hydrophobic residue.
New England Biolabs, Inc. EpiMark Hot Start Taq DNA Polymerase – 100 reactions
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA. Taq DNA polymerase possesses a 5- 3 polymerase activity (1,2,3) and a 5 flap endonuclease activity (4,5). EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
Zymo Research Corporation Atlantis dsDNase 12.5 U (0.1 U/ul)
Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
New England Biolabs, Inc. BstAPI – 200 units
A restriction endonuclease that recognizes the sequence GCAN_NNN^NTGC.